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walI [2019-07-16 10:25:19]
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walI [2019-07-16 10:25:19]

negative effector of [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK], controls cell wall metabolism
locus
BSU_40380
pI
5.28
mw
32.44 kDa
protein length
280 aa Sequence Blast
gene length
843 bp Sequence Blast
function
control of cell wall metabolism
product
negative effector of [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK]
essential
no
synonyms
yycI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.3|Control of response regulators/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    4,149,667 → 4,150,509

    Phenotypes of a mutant

  • growth defect at high temperature, lysis at 49°C, this can be suppressed by mutations that reduce [protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK] autokinase activity, by inactivation of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE], or by overexpreesion of [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|IseA] or [protein|603E226CE488A25C4E28A6A7363CCD65BE64BB21|PdaC] [pubmed|29465029]

    • The protein

    Structure

  • [PDB|2O3O], [Pubmed|17307848]

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot), spotty close to the membrane [Pubmed|21219466]

    • Expression and Regulation

    Operons

    genes
    [gene|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]-[gene|E88978809272FAC2520DB4ABCA0554F8028F3451|walK]-[gene|06D2D03C65D50E3909B2C93835193815B6016967|walH]-[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]-[gene|D5321F707E183E72B225F1CED2F2FDCB1183B88A|walJ]-[gene|75B8C37E589C855D2BAD7FB51B9600E161ACFBD3|htrC]
    description
    [Pubmed|9829949]

    regulation

  • ''htrC'' transcript expressed during sporulation [Pubmed|9829949]
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    Biological materials

    Mutant

  • MGNA-B825 (yycI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1824 NBRP B. subtilis, Japan]
  • BKE40380 (Δ[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCAACGATGAAGATTGATT, downstream forward: _UP4_TGAAAATAATGGAGTGAGAC
  • BKK40380 (Δ[gene|EE681C9A42FCC29CBB55CC9566EE4CFF986D0F1D|walI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCAACGATGAAGATTGATT, downstream forward: _UP4_TGAAAATAATGGAGTGAGAC

    • References

    Reviews

  • 28886686

    • Original publications

  • 17307850,17350627,17307848,9829949,18408157,21219466,29465029